Proliferating dendritic cell progenitors This may not be the complete list of references from this article. Selective ablation of proliferating microglial cells exacerbates ischemic injury in the brain Romani, N. Allogeneic primary MLR and ox- idative mitogenesis were performed exactly as described (29). 1084/jem. [Google Scholar] Bender A, Sapp M, Schuler G, Steinman RM, Bhardwaj N. Dec 1, 2001 · Fig. et al. S. Dec 1, 1992 · Therefore, GM-CSF generates all three lineages of myeloid cells (granulocytes, macrophages, and dendritic cells). Sep 1, 1998 · Identification of dendritic cell colony-forming units among normal human CD34+ bone-marrow progenitors that are expanded by c-kit-ligand and yield pure dendritic cell colonies in the presence of granulocyte/macrophage colony-stimulating factor and tumor necrosis factor α. Romani, Nikolaus; Gruner, Stefan; and Brang, Daniela, "Proliferating dendritic cell progenitors in human blood" (1994). . and the European Commission who supported B. By using the site you are agreeing to this as outlined in our Feb 1, 1999 · Dendritic cells (DC) have been described to develop along the myeloid and lymphoid lineage from pluripotent bone marrow stem cells (reviewed in Steinman, 1991; Cella et al. Selected References. The central column corresponds to the intermediate precursors or to immature DCs obtained after the first proliferative phase of differentiation [with the exception of pathway (e), which describes a single-step culture method cells cytospun or attached by poly-t-lysine to glass slides. Mouse myeloid DC progenitors with the ability to migrate through the blood and replenish the DC pool have been identified in bone marrow but the ontogeny of human DC is poorly Aug 8, 2023 · cDCs are short-lived cells that are constantly generated by bone marrow (BM) hematopoietic stem cells (HSCs) following a hierarchy of common myeloid progenitors (CMPs) → monocyte-DC progenitors (MDPs) → common DC progenitors (CDPs) → pre-DCs → DC1s and DC2s. 180. Proliferating dendritic cell Jan 1, 2020 · One LIN − HLA-DR + cluster with expression of myeloid-specific genes was identified as a cluster of myeloid cells. We find that aggregates of growing dendritic cells develop in cultures of mouse blood that are supplemented with granulocyte/macrophage colony-stimulating factor (GM-CSF) but not other CSFs. CD34+ cells are rare in adult blood, however, making it difficult to use CD34+ cells to ascertain if DC progenitors are present in the circulation and if blood can be a starting point to obtain large numbers of these immunostimulatory antigen-presenting cells for Jan 1, 2001 · CD34+ cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. Search life-sciences literature (41,978,575 articles, preprints and more) Jan 1, 2010 · Hematopoietic stem cells (HSC) in the bone marrow (BM) are long-lived, self-renewing precursors that undergo successive rounds of lineage commitment, giving rise to multi-potent myeloid, lymphoid, and erythroid progenitors. 2, 3 DC circulate in the blood in very small numbers, and migrate into Jul 1, 1994 · This website requires cookies, and the limited processing of your personal data in order to function. 9 KB). Jul 1, 1994 · Europe PMC is an archive of life sciences journal literature. The dendritic cell precursor derives from the Ia-negative and nonadherent fraction. Identification of proliferating dendritic cell precursors in mouse blood. CD34+cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. Feb 1, 1999 · We are very thankful to all the colleagues (see Section 2) that supplied us with valuable reagents. CD34+ cells are rare in adult blood, however, making it difficult to use CD34+ cells to ascertain if DC progenitors are present Sep 1, 1998 · DENDRITIC CELLS (DC) are derived from CD34 + progenitors in the bone marrow (BM). 83. 1. Historical Scientific Reports Full Text. This work was supported by a grant from the German Research Ministry (BMBF), Bonn, grant number 01 GE 9601 to G. Here, we describe culture conditions that, for the first time, allow the establishment of lymphoid-restricted, but uncommitted, long-term proliferating CLP cell … Jul 1, 1994 · Proliferating Dendritic Cell Progenitors in Human Blood Journal of Experimental Medicine - United States doi 10. Dendritic cells (DC) are professional phagocytes possessing a unique ability to sense perturbations in the tissue microenvironment and promote adaptive immune responses, whilst maintaining immunological tolerance. Lymphoid-related DC develop from very immature T-cell precursors or BM progenitors, which are also the source of future natural killer cells and B cells (2,3). Improved methods for the generation of dendritic cells from nonproliferating progenitors in human blood. These references are in PubMed. Despite the difficulties in purifying this trace Recommended Citation. 3, 5 DCs specifically depend on FLT3 and FLT3L for their differentiation and proliferation, 29, 30 whereas the development of May 1, 1992 · While it has been known that dendritic cells arise from proliferating precursors in situ, it has been difficult to identify progenitors in culture. Thurner in the Training and Mobility of Researchers (TMR) Programme `European Union Network for Investigation of Dendritic Cell Jul 1, 1994 · CD34+ cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. T Cell Stimulation Assays. Aug 20, 2015 · Human dendritic cells (DCs) develop from progressively restricted bone marrow (BM) progenitors: these progenitor cells include granulocyte, monocyte and DC progenitor (GMDP) cells; monocyte Large numbers of dendritic cells can be mobilized by specific cytokines from progenitors in the blood stream, and their use in stimulating T cell-mediated resistance to viruses and tumors should facilitate future studies of their FceRI and CD4 receptors. CD34+ cells are rare in adult blood, however, making it difficult to use CD34+ cells to ascertain if DC progenitors are present in the circulation and if blood can be a starting point to obtain large numbers of these immunostimulatory antigen-presenting cells for Proliferating dendritic cell progenitors in human blood Proliferating bipotential glial progenitor cells in adult rat optic nerve. CD34+cells are rare in adult blood, however, making it difficult to use CD34 + ceils to ascertain if DC progenitors are present in the circulation and if blood can be a starting point to obtain large numbers of these immunostimulatory antigenpresenting cells for Jul 1, 1994 · CD34+ cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. 84 Proliferating Dendritic Cell Progenitors in Human Blood Massive ex vivo generation of functional dendritic cells from mobilized CD34+ blood progenitors for anticancer therapy. 1 Human peripheral blood (PB) DC are closely related to monocytes. Exp Hematol. The Full Text of this article is available as a PDF (659. 1995 Dec; 23 (14):1463–1471. This feature should prove useful for future We report the generation of dendritic cells (DC) starting from CD34+ bone marrow (BM) progenitor cells, using a two-stage culture system in which, besides granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumour necrosis factor-alpha (TNF-alpha), stem-cell factor (SCF) was added during t … Dendritic cells (DC) form a system of antigen presenting cells that are specialized to stimulate resting T cells and to initiate T-dependent immune responses (“nature’s adjuvant”) [for review see 1]. Recent advances have been made in understanding their origin, their heterogeneity, the mechanism of antigen uptake, and the signals that induce their migration and maturation into immunostimulatory antigen-presenting cells. Since > 5 x 10(6) dendritic cells develop in 1 wk from precursors within the large hind limb bones of a single animal, marrow progenitors can act as a major source of dendritic cells. In vitro, these lymphoid-derived DC can be generated in the absence of granulocyte macrophage colony stimulating factor (GM-CSF), and are CD8 + and Fas ligand + (CD95L + ). , 1997). Upon reanalysis of this cluster, we identified three macrophage subsets, marked by high expression of CCR1, MARCO or TREM2; and six dendritic cell subsets, marked by high expression of CLEC9A, CXorf21, MCOLN2, LAMP3, KIAA0101 and Langerin, representing respectively cDC1, two Feb 1, 1997 · Dendritic cells are cells specialized for antigen capture, migration and T cell stimulation. CD34+ cells in human cord blood and marrow are known to give rise to dendritic cells (DC), as well as to other myeloid lineages. Jul 18, 2017 · Human XCR1+ dendritic cells derived in vitro from CD34+ progenitors closely resemble blood dendritic cells, including their adjuvant responsiveness, contrary to monocyte-derived dendritic cells Jul 1, 1994 · In cord blood, it was only necessary to deplete erythroid progenitors, and add granulocyte/macrophage colony-stimulating factor (GM-CSF) together with tumor necrosis factor (TNF), to observe many aggregates and the production of typical DC progeny. Their common precursor can be identified by hematopoietic progenitor colony-forming assay, and is derived from the common granulocyte-macrophage colony-forming unit (CFU-GM). 2 Main in vitro differentiation pathways of dendritic cells (DCs) in mice. May 3, 2018 · Common lymphoid progenitors (CLPs) differentiate to T and B lymphocytes, dendritic cells, natural killer cells, and innate lymphoid cells. For each differentiation pathway, precursor populations are defined in the left-hand column.